Notizie sullipo di IsoPlexis
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Notizie sullipo di IsoPlexis forex broker inc mt4 g3

Notizie sullipo di IsoPlexis

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Organizations from across the globe submitted their recent innovations for consideration in the BIG Innovation Awards. Nominations were judged by a select group of business leaders and executives who volunteered their time and expertise to score submissions. Last November, the company debuted its IsoSpark instrument, with a tabletop footprint that aims to provide single-cell proteomics capabilities to large and small laboratories alike.

IsoPlexis said it plans to begin shipments this month. The Single-Cell Intracellular Proteome solution from IsoPlexis grew out of several labs at Caltech, all seeking better ways to monitor protein-protein interactions in cancer cells with the goal of developing targeted treatments. With traditional methods such as Western blot, mass spectrometry, and flow cytometry, only a couple of protein types can be tracked at a given time.

With previous technology, phosphorylation was used to identify the function of the individual proteins, with no insight as to how they work together. The Single-Cell Intracellular Proteome reveals the function the same way, but is also able to provide the context of entire protein signaling pathways, uncovering how the network operates as a whole.

Understanding the entire network of cellular pathways allows researchers to better understand the downstream effects of aberrant cells, says Sean Mackay, CEO and cofounder of IsoPlexis. In cancers, he adds, this approach helps evaluate the efficacy of targeted treatments such as antibody therapies or small-molecule drugs. IsoPlexis has the ability to detect rare cell subset with superpowers highly functional cells which have been proven to be highly predictive biomarkers in studies across many different research areas.

This uniquely predictive functional biomarker is guiding the development of effective therapeutics for cancers, inflammation, and infectious diseases, all on an automated platform that harnesses the most powerful cells to personalize immune medicine. Single-cell proteomics firm IsoPlexis is looking to expand its market reach with the launch of a lower-priced, smaller-footprint platform and new assay capabilities.

The company also continues to move its technology into the clinic, having identified a blood-based biomarker of response to immunotherapy in melanoma that researchers presented on at the Society for Immunotherapy of Cancer SITC annual meeting this month.

The IsoSpark has an inch footprint and can run four chips at a time compared to eight chips simultaneously for the IsoLight system. Researchers urgently need new approaches for functional immune profiling in order to accelerate discovery and development of curative medicines. The newest innovations from IsoPlexis make functional proteomics widely accessible to virtually every laboratory, speeding development of personalized, curative medicines.

These systems detect the rare subsets of highly functional superpowered cells through measuring true function of the full range of cytokines, chemokines, and other proteins. All three systems leverage the same uniquely innovative applications in functional immune profiling, intracellular signaling omics, and high-plex automated immunoassays, accelerating therapeutic development.

The IsoSpark, a personalized proteomics system for any laboratory, makes unique functional proteomics accessible to every lab, providing an integrated and flexible solution for accelerating curative medicines. The IsoSpark, the IsoLight, and the IsoSpark Duo, along with functional immune landscaping, intracellular signaling omics, and high-plex automated immunoassays are the latest innovations from IsoPlexis.

The IsoSpark, introduced on November 11th, is currently available for pre-order in North America and Europe with shipping planned for January According to the company, the partners aim to identify markers of patient immune response to the virus that could be used to inform development of therapies and vaccines for the disease. Single-cell analysis is a relatively new technology that has revolutionized the ability of researchers to study variation within a population of cells e.

As sequencing technologies continue to advance, the IsoLight proteomics hub makes it possible to profile cells in multiple modalities on a single system—an advance that is making waves in cancer, infectious disease research, and beyond. Cancer cells develop resistance to targeted therapies, and their inherent functional heterogeneity makes it difficult for researchers to understand and characterize these resistance mechanisms.

Cellular state changes became prominent around day 3, as most probed analytes exhibited a sharp but transitory increase in variance. Understanding these functional adaptations allowed the team to predict and develop an effective therapeutic combination to overcome this adaptive resistance. Challenges remain in cell therapy manufacturing optimization and scalability, especially when developing autologous therapies. Combining the IsoLight system with the Cocoon platform allows quantification of the true functional biology of the cell product.

Although other analytic approaches provide rich data, they do not reveal single cell functional detail, which facilitates better predictions of potency, durability, and adverse effects. The Cocoon allows for centralized and decentralized manufacturing of novel cell therapies, and the IsoLight provides a solution for the functional characterization of the cell therapy product, to ensure correlative functional potency of the manufactured cell product, continues Mackay. The Branford, Connecticut-based company said it plans to use its single-cell proteomics platform to identify drivers of durable and protective immune responses to the virus, with the ultimate goal of using that information to inform therapeutics development.

Single-cell analysis sheds light on immune response to COVID infection, enables the rapid discovery of antibody leads, and points to ways to get ahead of future pandemics. Proteomic analysis can also fill in critical gaps concerning cell type and function.

A Seattle-based research consortium headed by the Swedish Medical Center, the Institute for Systems Biology and Merck is also applying a host of multi-omic analytical techniques to blood specimens collected from patients with COVID, including a platform developed by IsoPlexis that employs microchip-based immunoassays to profile secreted cytokines and other signaling molecules from individual immune cells.

FlowMetric Life Sciences, Inc. This certification was awarded after FlowMetric met the rigorous training and testing requirements set out by IsoPlexis. FlowMetric can now provide Single-Cell Functional Proteomic services to its Pharmaceutical and Biotechnology customers to complement its decade-long expertise in developing and validating multi-parameter Flow Cytometry-based approaches for drug development.

The IsoPlexis technology provides comprehensive workflows that combine proprietary content and bioinformatic solutions to enable large data sets to be acquired from small sample volumes. Automate your entire workflow with one system. Just add your sample and walk away, achieving fully analyzed data on the same day. No additional equipment is needed. That means no washing and incubation stations, no centrifuge, no vortexer, and no plate reader. All of your proteomic needs are integrated into the completely automated IsoLight system.

For years, the relative abundance of particular proteins has been estimated from mRNAs in bulk tissue samples. Single-cell transcriptomics allowed a more pinpointed inference of protein levels based on detected RNA transcripts. But now the nascent field of single-cell proteomics promises direct measurements of the proteins within or secreted from individual cells. IsoPlexis offers the IsoLight platform for highly multiplexed single-cell proteomics of over 30 cytokines released from individual cells.

Applications of their technology are helping to predict which patients might respond to particular therapies for blood and solid tumor cancers as well as infectious disease, says Sean Mackay, co-founder and CEO of IsoPlexis. IsoPlexis is developing assays on its IsoLight system for detecting the inflammatory immune response generated by cells including peripheral monocytes, highly polyfunctional secretomic T cells, and microglia and exploring whether these profiles could be used for diagnosing and monitoring inflammatory neurodegenerative diseases.

Vaccine development can be daunting. GEN spoke to five leading researchers to hear their views and strategies on infectious disease research and vaccine development. Joseph Health network. Single-cell proteomics firm IsoPlexis has to date largely marketed its IsoLight platform to researchers doing work on cancer immunotherapy, where it has proved useful for assessing immune cell cytokine production.

Given the apparently important role of overactive cytokine production, the so-called cytokine storm, in COVID deaths, the company is now working with researchers at the Institute for Systems Biology in Seattle to analyze the cytokine production of immune cells taken from COVID patients.

The partners said they plan to carry out research on immune cells from people who have been diagnosed with, or recovered from, COVID According to IsoPlexis, uncovering functional immune responses using its technologies has led to key breakthroughs in therapies that harness the immune system across disease areas. Heath, PhD. Single-cell proteomics firm IsoPlexis is collaborating with researchers at the Institute for Systems Biology in Seattle to look at the immune response in patients who have been diagnosed or recovered from COVID, with the goal of better understanding what factors are involved in better and worse outcomes.

The team aims to examine a variety of immune cell types—including various T cells and myeloid cells—to put together a map of the overall response. IsoPlexis has released two new products for use with its IsoLight cellular proteomics instrument. CodePlex Secretome is a fully automated approach to obtaining highly multiplexed bulk cytokine data that requires five minutes of hands-on time for sample loading. CodePlex Secretome multiplexes more than 30 cytokines, and is modular so users can simultaneously examine as few as eight samples or as many as It joins other IsoLight single-cell chip applications which allow researchers to visualize single-cell-level data and pinpoint biological drivers.

Unable to be detected by conventional technologies, cellular functional heterogeneity leads to differences in patient response and disease progression. By exploring, understanding, and characterizing cells based on function, researchers can accelerate therapeutic development by revealing new phenotypes that specifically correlate to individual disease or response states.

In a recent study led by researchers at the University of California, Los Angeles, proteomics firm IsoPlexis demonstrated the capabilities of its expanded single-cell analysis platform. The company is also releasing this quarter a panel aimed at bulk cell analysis of cytokines. While IsoPlexis has focused primarily on single-cell analysis, Mackay said it has received requests from customers interested in doing comparisons of single-cell and bulk cytokine profiles.

Nektar Therapeutics today announced the publication of preclinical data on its lead immuno-oncology candidate, NKTR , bempegaldesleukin bempeg in two manuscripts in Nature Communications. The published data demonstrate that bempeg, in combination with immune-based therapies including checkpoint inhibition CPI , antigen-specific vaccination and adoptive cell transfer ACT therapy, enhanced T-cell mediated tumor control by selectively expanding effector T cells Teffs over T regulatory cells Tregs in the tumor microenvironment.

In the second manuscript, published this week in Nature Communications , a research team led by Antoni Ribas , Ph. The studies found ACT therapy supported by bempeg increases the proliferation, homing and persistence of anti-tumor T cells compared to ACT with IL-2, resulting in superior antitumor activity in the B16 melanoma model.

Immuno-oncology combination for melanoma using Nektar drug boosts cell therapy in mice. The UCLA team is planning future studies to better understand how signaling pathways contribute to the anti-cancer effects they observed in mice given the T-cell combination therapy, they wrote in the study. To view the on demand webinar, visit here. The last decade was an eventful one for proteomics as the field saw a number of advances ranging from new workflows and applications to improvements in instrumentation and bioinformatic innovations.

Immunoassay-based methods aimed at measuring relatively large numbers of proteins at the single-cell level also saw significant advances during the decade. This was particularly true on the commercial side, with companies including Fluidigm, NanoString Technologies, IsoPlexis, Akoya Biosciences, Zellkraftwerk, IonPath, and Mission Bio launching tools for single-cell protein analysis during the last decade.

After two-plus decades spent struggling to drive experiment run times down to more manageable levels, a sense emerged this year within the field that this challenge had at last been met. Single-cell proteomic technologies also saw continued growth with a number of new developments in what is becoming an increasingly crowded field. According to the company, its technology has been used in a number of different settings including precision drug discovery and biomarker discovery in oncology, to identify proteomic differences that are often undetectable via other methods.

The company said it plans to use the funds to drive commercialization of its IsoLight single-cell proteomic system. Leonardo Ferreira, a postdoctoral fellow at the University of California, San Francisco, discusses the application of single cell proteomics data to understanding the function and therapeutic potential of genetically engineered regulatory T cells.

Understanding T-cell polyfunctionality: How single cell proteomics data drive CAR-T cell therapy research and development. Vladimir Senyukov, Director of BioAnalytical Development at Precision Biosciences, talks about investigating T-cell cytokine production using single cell proteomics in order to unlock the therapeutic potential of allogeneic CAR-T cells. IsoPlexis reported today that it has launched IsoSpeak software for automated on-site analysis and advanced, functional, single-cell mapping.

This information is critical for developing immunotherapy applications. Our software takes all that information and those multi-data points and condenses it in your hand in an easy-to-use fashion. Getting the data is quick, and once the run is done, all those data visualizations are able to be manipulated by users on the same day. IsoPlexis also expanded its applications with new IsoCode Chip products, including the murine cell Single Cell Polyfunctional Strength solution to open up a variety of previously published insights in the pre-clinical realm.

Additionally, the Single Cell Polyfunctional Inflammation solution helps discover and target important inflammatory cell types, in high need areas like auto-immune disease, neuro-inflammation, and immune related adverse events. With a deeper understanding of cellular function by characterizing the functional cytokines from each single cell, scientists can analyze how the CAR-T cells are likely to impact the immune system, which can translate to how the therapy would affect patient outcome.

IsoPlexis, which recently received an NCI grant, reports that it has published a technology note with Miltenyi Biotec in a co-marketing agreement. IsoPlexis said today that it has entered an exclusive agreement with BioStream to distribute its single-cell protein analysis products in Japan. Optimizing Immunotherapy Lead Selection.

An urgent need exists for novel detection strategies to better understand mechanistic function and efficacy at the single-cell level in a way that correlates to clinical outcomes. Population level studies do not provide the necessary insight into immune response heterogeneity at the single-cell level. There are no currently well-defined methods to predict or characterize adverse effects, such as severe cytokine release syndrome CRS or neurotoxicity.

In addition, the CAR T-cell infusion product may contain some cells with bizarre functions. Signs, Portents, and Immuno-Oncology Biomarkers. For example, cytokine secretion profiles correlate with, and can help predict, clinical outcomes in immunotherapy, said Will Singleterry, PhD, director of business development at IsoPlexis. Researchers and clinicians urgently need new approaches to understand the functional profile of cell therapy products to accelerate discovery and development, to achieve manufacturing consistency, and to predict which patients will derive the most value from these drugs while minimizing side-effects.

Having the ability to drive better outcomes by profiling the complete function of each cell would be a significant advantage for bioengineers—a challenge the IsoPlexis single-cell proteomic system has been designed to address. James Heath, PhD.

There is an urgent need to characterize the potency and efficacy of CRISPR-Cas9-modified inducible pluripotent stem cell-derived natural killer cells for preclinical cancer immunotherapy research. In work presented at the recent American Association for Cancer Research annual meeting, they found that T-cell cytokine production predicted patient response. The Branford, Connecticut-based company said that it will use the funds to commercialize its IsoLight platform for use in quality control and guidance of CAR T therapy.

CAR T cells find strength in polyfunction. In this issue of Blood , Rossi et al report that the heterogeneity of personalized chimeric antigen receptor CAR T cells has limited the ability to identify product attributes that enhance patient outcomes, but they have showed that by using single-cell analyses, heterogeneity may actually improve clinical outcomes. Single-cell polyfunctional analyses provide a measure of product efficacy that can be used to identify patients likely to respond to treatment and those likely to develop toxicities.

As IsoPlexis prepares to take its production to the next level in , two science news publications have honored the company for technology that tailors optimum treatments for cancer patients. Congratulations to IsoPlexis director and co-inventor Dr.

Sales and Marketing expenses as a percentage of total revenue have risen sharply as revenues have increased, as the figures below indicate:. The Sales and Marketing efficiency rate, defined as how many dollars of additional new revenue are generated by each dollar of Sales and Marketing spend, was stable at 0.

Also, below is a chart showing the global single cell analysis market in , by end-use type". Below are relevant financial results derived from the firm's registration statement:. Glossary Of Terms. Excluding effects of underwriter options and private placement shares or restricted stock, if any, the float to outstanding shares ratio will be approximately Management says it will use the net proceeds from the IPO as follows:.

We intend to use the net proceeds from this offering for general corporate purposes, including working capital, research and development, sales and marketing activities, general administrative matters, operating expenses and capital expenditures. Management's presentation of the company roadshow is available here.

Regarding outstanding legal proceedings, management says the firm is not subject to any legal actions that it believes would have a material adverse effect on its financial condition or operations. Below is a table of relevant capitalization and valuation figures for the company:.

As a reference, a potential partial public comparable to IsoPlexis would be Bio-Rad Laboratories BIO ; below is a comparison of their primary valuation metrics:. Bio-Rad BIO. IsoPlexis ISO. IsoPlexis is seeking public capital market investment to fund its continued ramp up of commercializing its cell analysis platform.

The firm's financials show a high growth rate in topline revenue, strong gross profit increase, but downward trending gross margin, increasing operating and net losses and growing cash used in operations. Sales and Marketing expenses as a percentage of total revenue have risen sharply as revenue has increased; its Sales and Marketing efficiency rate was stable at 0.

Morgan Stanley is the lead underwriter and IPOs led by the firm over the last month period have generated an average return of This is a mid-tier performance for all major underwriters during the period. The primary risk to the company's outlook is its ability to continue to penetrate its target markets against large, entrenched competitors. Gain Insight and actionable information on U.

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The newest innovations from IsoPlexis make functional proteomics widely accessible to virtually every laboratory, speeding development of personalized, curative medicines. These systems detect the rare subsets of highly functional superpowered cells through measuring true function of the full range of cytokines, chemokines, and other proteins.

All three systems leverage the same uniquely innovative applications in functional immune profiling, intracellular signaling omics, and high-plex automated immunoassays, accelerating therapeutic development. The IsoSpark, a personalized proteomics system for any laboratory, makes unique functional proteomics accessible to every lab, providing an integrated and flexible solution for accelerating curative medicines.

The IsoSpark, the IsoLight, and the IsoSpark Duo, along with functional immune landscaping, intracellular signaling omics, and high-plex automated immunoassays are the latest innovations from IsoPlexis. The IsoSpark, introduced on November 11th, is currently available for pre-order in North America and Europe with shipping planned for January According to the company, the partners aim to identify markers of patient immune response to the virus that could be used to inform development of therapies and vaccines for the disease.

Single-cell analysis is a relatively new technology that has revolutionized the ability of researchers to study variation within a population of cells e. As sequencing technologies continue to advance, the IsoLight proteomics hub makes it possible to profile cells in multiple modalities on a single system—an advance that is making waves in cancer, infectious disease research, and beyond.

Cancer cells develop resistance to targeted therapies, and their inherent functional heterogeneity makes it difficult for researchers to understand and characterize these resistance mechanisms. Cellular state changes became prominent around day 3, as most probed analytes exhibited a sharp but transitory increase in variance.

Understanding these functional adaptations allowed the team to predict and develop an effective therapeutic combination to overcome this adaptive resistance. Challenges remain in cell therapy manufacturing optimization and scalability, especially when developing autologous therapies. Combining the IsoLight system with the Cocoon platform allows quantification of the true functional biology of the cell product.

Although other analytic approaches provide rich data, they do not reveal single cell functional detail, which facilitates better predictions of potency, durability, and adverse effects. The Cocoon allows for centralized and decentralized manufacturing of novel cell therapies, and the IsoLight provides a solution for the functional characterization of the cell therapy product, to ensure correlative functional potency of the manufactured cell product, continues Mackay.

The Branford, Connecticut-based company said it plans to use its single-cell proteomics platform to identify drivers of durable and protective immune responses to the virus, with the ultimate goal of using that information to inform therapeutics development. Single-cell analysis sheds light on immune response to COVID infection, enables the rapid discovery of antibody leads, and points to ways to get ahead of future pandemics.

Proteomic analysis can also fill in critical gaps concerning cell type and function. A Seattle-based research consortium headed by the Swedish Medical Center, the Institute for Systems Biology and Merck is also applying a host of multi-omic analytical techniques to blood specimens collected from patients with COVID, including a platform developed by IsoPlexis that employs microchip-based immunoassays to profile secreted cytokines and other signaling molecules from individual immune cells.

FlowMetric Life Sciences, Inc. This certification was awarded after FlowMetric met the rigorous training and testing requirements set out by IsoPlexis. FlowMetric can now provide Single-Cell Functional Proteomic services to its Pharmaceutical and Biotechnology customers to complement its decade-long expertise in developing and validating multi-parameter Flow Cytometry-based approaches for drug development.

The IsoPlexis technology provides comprehensive workflows that combine proprietary content and bioinformatic solutions to enable large data sets to be acquired from small sample volumes. Automate your entire workflow with one system. Just add your sample and walk away, achieving fully analyzed data on the same day. No additional equipment is needed. That means no washing and incubation stations, no centrifuge, no vortexer, and no plate reader.

All of your proteomic needs are integrated into the completely automated IsoLight system. For years, the relative abundance of particular proteins has been estimated from mRNAs in bulk tissue samples. Single-cell transcriptomics allowed a more pinpointed inference of protein levels based on detected RNA transcripts.

But now the nascent field of single-cell proteomics promises direct measurements of the proteins within or secreted from individual cells. IsoPlexis offers the IsoLight platform for highly multiplexed single-cell proteomics of over 30 cytokines released from individual cells. Applications of their technology are helping to predict which patients might respond to particular therapies for blood and solid tumor cancers as well as infectious disease, says Sean Mackay, co-founder and CEO of IsoPlexis.

IsoPlexis is developing assays on its IsoLight system for detecting the inflammatory immune response generated by cells including peripheral monocytes, highly polyfunctional secretomic T cells, and microglia and exploring whether these profiles could be used for diagnosing and monitoring inflammatory neurodegenerative diseases. Vaccine development can be daunting. GEN spoke to five leading researchers to hear their views and strategies on infectious disease research and vaccine development.

Joseph Health network. Single-cell proteomics firm IsoPlexis has to date largely marketed its IsoLight platform to researchers doing work on cancer immunotherapy, where it has proved useful for assessing immune cell cytokine production. Given the apparently important role of overactive cytokine production, the so-called cytokine storm, in COVID deaths, the company is now working with researchers at the Institute for Systems Biology in Seattle to analyze the cytokine production of immune cells taken from COVID patients.

The partners said they plan to carry out research on immune cells from people who have been diagnosed with, or recovered from, COVID According to IsoPlexis, uncovering functional immune responses using its technologies has led to key breakthroughs in therapies that harness the immune system across disease areas. Heath, PhD. Single-cell proteomics firm IsoPlexis is collaborating with researchers at the Institute for Systems Biology in Seattle to look at the immune response in patients who have been diagnosed or recovered from COVID, with the goal of better understanding what factors are involved in better and worse outcomes.

The team aims to examine a variety of immune cell types—including various T cells and myeloid cells—to put together a map of the overall response. IsoPlexis has released two new products for use with its IsoLight cellular proteomics instrument.

CodePlex Secretome is a fully automated approach to obtaining highly multiplexed bulk cytokine data that requires five minutes of hands-on time for sample loading. CodePlex Secretome multiplexes more than 30 cytokines, and is modular so users can simultaneously examine as few as eight samples or as many as It joins other IsoLight single-cell chip applications which allow researchers to visualize single-cell-level data and pinpoint biological drivers.

Unable to be detected by conventional technologies, cellular functional heterogeneity leads to differences in patient response and disease progression. By exploring, understanding, and characterizing cells based on function, researchers can accelerate therapeutic development by revealing new phenotypes that specifically correlate to individual disease or response states. In a recent study led by researchers at the University of California, Los Angeles, proteomics firm IsoPlexis demonstrated the capabilities of its expanded single-cell analysis platform.

The company is also releasing this quarter a panel aimed at bulk cell analysis of cytokines. While IsoPlexis has focused primarily on single-cell analysis, Mackay said it has received requests from customers interested in doing comparisons of single-cell and bulk cytokine profiles.

Nektar Therapeutics today announced the publication of preclinical data on its lead immuno-oncology candidate, NKTR , bempegaldesleukin bempeg in two manuscripts in Nature Communications. The published data demonstrate that bempeg, in combination with immune-based therapies including checkpoint inhibition CPI , antigen-specific vaccination and adoptive cell transfer ACT therapy, enhanced T-cell mediated tumor control by selectively expanding effector T cells Teffs over T regulatory cells Tregs in the tumor microenvironment.

In the second manuscript, published this week in Nature Communications , a research team led by Antoni Ribas , Ph. The studies found ACT therapy supported by bempeg increases the proliferation, homing and persistence of anti-tumor T cells compared to ACT with IL-2, resulting in superior antitumor activity in the B16 melanoma model. Immuno-oncology combination for melanoma using Nektar drug boosts cell therapy in mice.

The UCLA team is planning future studies to better understand how signaling pathways contribute to the anti-cancer effects they observed in mice given the T-cell combination therapy, they wrote in the study. To view the on demand webinar, visit here.

The last decade was an eventful one for proteomics as the field saw a number of advances ranging from new workflows and applications to improvements in instrumentation and bioinformatic innovations. Immunoassay-based methods aimed at measuring relatively large numbers of proteins at the single-cell level also saw significant advances during the decade. This was particularly true on the commercial side, with companies including Fluidigm, NanoString Technologies, IsoPlexis, Akoya Biosciences, Zellkraftwerk, IonPath, and Mission Bio launching tools for single-cell protein analysis during the last decade.

After two-plus decades spent struggling to drive experiment run times down to more manageable levels, a sense emerged this year within the field that this challenge had at last been met. Single-cell proteomic technologies also saw continued growth with a number of new developments in what is becoming an increasingly crowded field. According to the company, its technology has been used in a number of different settings including precision drug discovery and biomarker discovery in oncology, to identify proteomic differences that are often undetectable via other methods.

The company said it plans to use the funds to drive commercialization of its IsoLight single-cell proteomic system. Leonardo Ferreira, a postdoctoral fellow at the University of California, San Francisco, discusses the application of single cell proteomics data to understanding the function and therapeutic potential of genetically engineered regulatory T cells. Understanding T-cell polyfunctionality: How single cell proteomics data drive CAR-T cell therapy research and development.

Vladimir Senyukov, Director of BioAnalytical Development at Precision Biosciences, talks about investigating T-cell cytokine production using single cell proteomics in order to unlock the therapeutic potential of allogeneic CAR-T cells. IsoPlexis reported today that it has launched IsoSpeak software for automated on-site analysis and advanced, functional, single-cell mapping. This information is critical for developing immunotherapy applications.

Our software takes all that information and those multi-data points and condenses it in your hand in an easy-to-use fashion. Getting the data is quick, and once the run is done, all those data visualizations are able to be manipulated by users on the same day. IsoPlexis also expanded its applications with new IsoCode Chip products, including the murine cell Single Cell Polyfunctional Strength solution to open up a variety of previously published insights in the pre-clinical realm.

Additionally, the Single Cell Polyfunctional Inflammation solution helps discover and target important inflammatory cell types, in high need areas like auto-immune disease, neuro-inflammation, and immune related adverse events. With a deeper understanding of cellular function by characterizing the functional cytokines from each single cell, scientists can analyze how the CAR-T cells are likely to impact the immune system, which can translate to how the therapy would affect patient outcome.

IsoPlexis, which recently received an NCI grant, reports that it has published a technology note with Miltenyi Biotec in a co-marketing agreement. IsoPlexis said today that it has entered an exclusive agreement with BioStream to distribute its single-cell protein analysis products in Japan. Optimizing Immunotherapy Lead Selection. An urgent need exists for novel detection strategies to better understand mechanistic function and efficacy at the single-cell level in a way that correlates to clinical outcomes.

Population level studies do not provide the necessary insight into immune response heterogeneity at the single-cell level. There are no currently well-defined methods to predict or characterize adverse effects, such as severe cytokine release syndrome CRS or neurotoxicity. In addition, the CAR T-cell infusion product may contain some cells with bizarre functions.

Signs, Portents, and Immuno-Oncology Biomarkers. For example, cytokine secretion profiles correlate with, and can help predict, clinical outcomes in immunotherapy, said Will Singleterry, PhD, director of business development at IsoPlexis. Researchers and clinicians urgently need new approaches to understand the functional profile of cell therapy products to accelerate discovery and development, to achieve manufacturing consistency, and to predict which patients will derive the most value from these drugs while minimizing side-effects.

Having the ability to drive better outcomes by profiling the complete function of each cell would be a significant advantage for bioengineers—a challenge the IsoPlexis single-cell proteomic system has been designed to address. James Heath, PhD. There is an urgent need to characterize the potency and efficacy of CRISPR-Cas9-modified inducible pluripotent stem cell-derived natural killer cells for preclinical cancer immunotherapy research. In work presented at the recent American Association for Cancer Research annual meeting, they found that T-cell cytokine production predicted patient response.

The Branford, Connecticut-based company said that it will use the funds to commercialize its IsoLight platform for use in quality control and guidance of CAR T therapy. CAR T cells find strength in polyfunction. In this issue of Blood , Rossi et al report that the heterogeneity of personalized chimeric antigen receptor CAR T cells has limited the ability to identify product attributes that enhance patient outcomes, but they have showed that by using single-cell analyses, heterogeneity may actually improve clinical outcomes.

Single-cell polyfunctional analyses provide a measure of product efficacy that can be used to identify patients likely to respond to treatment and those likely to develop toxicities. As IsoPlexis prepares to take its production to the next level in , two science news publications have honored the company for technology that tailors optimum treatments for cancer patients.

Congratulations to IsoPlexis director and co-inventor Dr. Lee is a giant of science, and he has changed the face of modern biology. Through scholarship, innovation, and an outstanding faculty, he has also built ISB into the world leader of systems biology.

The data show the potential to predict whether cancer patients will respond to CAR-T cell therapy prior to treatment, as well as to improve both pre-infusion potency testing and cell product design. Keytruda success a crowning moment for cancer immunotherapies, but are more on their way?

The licensing deals, mergers and acquisitions, and excitement at events such as ASCO in the last couple of years, have of course come in combination with the impressive data that clinical trials are yielding, notes Sean Mackay, the chief executive of IsoPlexis. Its partners include the cancer immunotherapy specialist, Kite Pharma.

Omics firm IsoPlexis is developing an automated version of its single-cell analysis platform for use in large clinical trials of CAR-T therapies for blood cancers. That means before treating a patient, doctors would be able to tell whether the immunotherapy would be effective and whether it would cause unpleasant side effects. A Branford life sciences company is working to take the guesswork out of cancer treatments with technology that may predict how patients will respond to drugs before being exposed to them.

This new single-cell technology allows researchers to characterize cells based on the proteins they secrete—as many as 42 different cytokines, chemokines, and other molecule types at once. The Life Sciences Innovation Report showcases outstanding innovation that is driving improvements and transforming the industry. A UCLA study in collaboration with the California Institute of Technology Caltech shows that skin cancer cells could be chemically changed from within to reflexively alter gene expression patterns and intracellular pathways, which allows the cells to become resistant to targeted drugs.

Researchers at this Center have applied single cell proteomics studies towards understanding patient responses to immunotherapy in clinical trials. This technology has been translated into the commercial sector for cancer immunotherapy applications by IsoPlexis, a company co-founded by former NSBCC postdoc Dr. Toni Ribas. A team led by researchers at the California Institute of Technology and the University of California, San Diego, has used single-cell phosphoproteomics to detect changes in protein signaling linked to the development of drug resistance in glioblastoma.

Below is a table of relevant capitalization and valuation figures for the company:. As a reference, a potential partial public comparable to IsoPlexis would be Bio-Rad Laboratories BIO ; below is a comparison of their primary valuation metrics:. Bio-Rad BIO. IsoPlexis ISO. IsoPlexis is seeking public capital market investment to fund its continued ramp up of commercializing its cell analysis platform. The firm's financials show a high growth rate in topline revenue, strong gross profit increase, but downward trending gross margin, increasing operating and net losses and growing cash used in operations.

Sales and Marketing expenses as a percentage of total revenue have risen sharply as revenue has increased; its Sales and Marketing efficiency rate was stable at 0. Morgan Stanley is the lead underwriter and IPOs led by the firm over the last month period have generated an average return of This is a mid-tier performance for all major underwriters during the period. The primary risk to the company's outlook is its ability to continue to penetrate its target markets against large, entrenched competitors.

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I wrote this article myself, and it expresses my own opinions. I am not receiving compensation for it other than from Seeking Alpha. I have no business relationship with any company whose stock is mentioned in this article. Additional disclosure: Investing in IPOs is an inherently volatile and opaque endeavor.

I analyze fundamental company performance and my conclusions may not be relevant for first-day or early IPO trading activity, which can be highly volatile and unrelated to company fundamentals. This report is intended for educational purposes only and is not financial, legal or investment advice. Donovan Jones Marketplace. Customer Acquisition The firm seeks customers among biopharmaceutical firms, major cancer medical centers and researchers worldwide.

Sales and Marketing expenses as a percentage of total revenue have risen sharply as revenues have increased, as the figures below indicate: Sales and Marketing Expenses vs. Revenue Period Percentage Six Mos. Ended June 30, Ended June 30, 0.